Development of the marrellid arthropod from the Fezouata Shale

Today’s arthropods –critters with jointed legs that contain today’s crabs, beetles, or spiders– often show very complex development with their juveniles and larvae living and feeding in a different way than the adults. A classical example is a flying butterfly with an edacious caterpillar or a sessile barnacle with a floating larva. But was such a complex kind of development present in the early members of Arthropoda?

Our international team of researchers led by Lukas Laibl of the Institute of Geology of the Czech Academy of Sciences and Allison C. Daley of the University of Lausanne tackled the question by studying a few millimeters-long larvae of 480 million years old arthropod species from Morocco belonging to an extinct group called marrellids, which thrived in early Paleozoic seas. The results are presented in a paper published today in Frontiers in Ecology and Evolution.

Adult and early developmental stage of Fezouata marrellid. Adult specimen MGL 102397, immature specimen MGL 102382, shown at the same scale.

To better understand the anatomy of this Paleozoic animal, we imaged the tiniest larvae using high-resolution synchrotron-based computed tomography (CT) scanning at the Paul Scherrer Institut in Switzerland. My main contribution to this work was meticulously extracting and rendering in three dimensions the morphological details preserved in this 2-mm-long specimen, from larger anatomical features to micrometer-scale details, including tiny claws and delicate hairs on its legs.

3D rendering of the 2-mm-long marrellid larva from the Fezouata Shale of Morocco. Color key: white, body; gold, spines; brown, antennae; green, second pair of cephalic appendages; blue, walking limbs; pink, food-gathering limbs; grey, gills.

Detailed examination of the appendages of this early arthropod larvae tells a lot about its mode of life. On the head, it had sensory antennae, a pair of robust legs used to orientate its body or to anchor itself on the sediment, and a pair of walking limbs. Two additional pairs of legs on the trunk were also used for walking, while the rear limbs of the trunk bore spines and delicate hairs and were used for capturing small organic particles that the animal was eating. All trunk limbs also had a gill branch used for respiration.

Interestingly the adult stages of various marrellids seem to have a very similar mode of life as these tiny babies, contrary to what is seen in many recent arthropods. We also show that the tiny larvae even lived in the same locations and environment as the adults of the same species, on the seafloor at the margin of an ancient continent called Gondwana, just below the storm wave base.

Marrellids are considered to be very early arthropods, showing many ancestral features. We therefore think that such development, with no major change in feeding and ecology between larvae and adults, was ancestral for arthropods as a whole. Other ancient arthropod groups present a similar development, thus supporting our conclusions. This indicates that complex life cycles must have evolved independently later in several arthropod groups.

Reference: Laibl L., Gueriau P., Saleh F, Pérez-Peris F., Lustri L., Drage H.B., Bath Enright O.G., Potin G. & Daley A.C. 2023. Early developmental stages of a Lower Ordovician marrellid from Morocco suggest simple ontogenetic niche differentiation in early euarthropods. Frontiers in Ecology and Evolution 11, 1232612. Find the article (Open Access) here